MersV1, Main, Exploration, bibRecord, 004865

Site-specific interaction of the antitumor antibiotic dynemicin with branched DNA molecules.

Identifieur interne : 004865 ( Main/Exploration ); précédent : 004864; suivant : 004866

Site-specific interaction of the antitumor antibiotic dynemicin with branched DNA molecules.

Auteurs : M. Lu [États-Unis] ; Q. Guo ; N R Kallenbach

Source :

Journal of biomolecular structure & dynamics [ 0739-1102 ] ; 1991.

RBID : pubmed:1741963

Descripteurs français

KwdFr :
- ADN (), ADN (métabolisme), Anthraquinones (), Anthraquinones (métabolisme), Antibiotiques antinéoplasiques (), Antibiotiques antinéoplasiques (métabolisme), Données de séquences moléculaires, Sites de fixation, Structure moléculaire, Séquence nucléotidique, Ènediynes.
MESH :
- métabolisme : ADN, Anthraquinones, Antibiotiques antinéoplasiques.
- ADN, Anthraquinones, Antibiotiques antinéoplasiques, Données de séquences moléculaires, Sites de fixation, Structure moléculaire, Séquence nucléotidique, Ènediynes.

English descriptors

KwdEn :
- Anthraquinones (chemistry), Anthraquinones (metabolism), Antibiotics, Antineoplastic (chemistry), Antibiotics, Antineoplastic (metabolism), Base Sequence, Binding Sites, DNA (chemistry), DNA (metabolism), Enediynes, Molecular Sequence Data, Molecular Structure.
MESH :
- chemical , chemistry : Anthraquinones, Antibiotics, Antineoplastic, DNA.
- chemical , metabolism : Anthraquinones, Antibiotics, Antineoplastic, DNA.
- Base Sequence, Binding Sites, Enediynes, Molecular Sequence Data, Molecular Structure.

Abstract

A specific interaction of stable branched DNA molecules with the antitumor antibiotic dynemicin is reported. Dynemicin contains an anthraquinone and an enediyne unit, and belongs to the family of enediyne antitumor agents. DNA strand scission by dynemicin appears to involve interaction of the anthraquinone core with DNA and release of a phenyl diradical from the enediyne core that can abstract hydrogen atoms from the sugar phosphate backbone of DNA. The cleavage patterns of each labeled strand in two branched tetramers of four 16-mers are compared with those of the same strands in unbranched duplex controls. Differences between the profiles corresponding to scission of branched and duplex DNA molecules can be detected in most of the strands. The strongest differences define a specific site flanking the branch in each of two branched structures. At 18 degrees C, cleavage at strand positions demarcating the site of enhanced affinity in both junctions is observed to be 70-100% more efficient than at the corresponding sequence positions in the control duplex DNA molecules. The patterns of preferential cleavage at these sites are significantly altered in the presence of excess propidium diiodide, an intercalative drug.

DOI: 10.1080/07391102.1991.10507912
PubMed: 1741963

Affiliations:

Le document en format XML

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<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">État de New York</region>
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<wicri:cityArea>Department of Chemistry, New York University</wicri:cityArea>
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<term>Antibiotics, Antineoplastic (metabolism)</term>
<term>Base Sequence</term>
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<term>DNA (metabolism)</term>
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<term>Molecular Structure</term>
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<term>Anthraquinones (métabolisme)</term>
<term>Antibiotiques antinéoplasiques ()</term>
<term>Antibiotiques antinéoplasiques (métabolisme)</term>
<term>Données de séquences moléculaires</term>
<term>Sites de fixation</term>
<term>Structure moléculaire</term>
<term>Séquence nucléotidique</term>
<term>Ènediynes</term>
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<term>Antibiotics, Antineoplastic</term>
<term>DNA</term>
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<term>Antibiotics, Antineoplastic</term>
<term>DNA</term>
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<term>Antibiotiques antinéoplasiques</term>
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<term>Binding Sites</term>
<term>Enediynes</term>
<term>Molecular Sequence Data</term>
<term>Molecular Structure</term>
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<front><div type="abstract" xml:lang="en">A specific interaction of stable branched DNA molecules with the antitumor antibiotic dynemicin is reported. Dynemicin contains an anthraquinone and an enediyne unit, and belongs to the family of enediyne antitumor agents. DNA strand scission by dynemicin appears to involve interaction of the anthraquinone core with DNA and release of a phenyl diradical from the enediyne core that can abstract hydrogen atoms from the sugar phosphate backbone of DNA. The cleavage patterns of each labeled strand in two branched tetramers of four 16-mers are compared with those of the same strands in unbranched duplex controls. Differences between the profiles corresponding to scission of branched and duplex DNA molecules can be detected in most of the strands. The strongest differences define a specific site flanking the branch in each of two branched structures. At 18 degrees C, cleavage at strand positions demarcating the site of enhanced affinity in both junctions is observed to be 70-100% more efficient than at the corresponding sequence positions in the control duplex DNA molecules. The patterns of preferential cleavage at these sites are significantly altered in the presence of excess propidium diiodide, an intercalative drug.</div>
</front>
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Serveur d'exploration MERS

Site-specific interaction of the antitumor antibiotic dynemicin with branched DNA molecules.

Site-specific interaction of the antitumor antibiotic dynemicin with branched DNA molecules.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration MERS
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